Western Blot of Goat anti-Rabbit IgG Peroxidase Conjugated Antibody. Lane 1: HeLa Whole Cell Lysate. Lane 2: NIH 3T3 Whole Cell Lysate. Load: 10 μg per lane. Primary antibody: Beta Actin antibody at 1:2,000 for overnight at 4°C. Secondary antibody (2 Blots on Left): Atto 647N goat secondary antibody at 1:10,000 for 60 min at RT. Secondary Antibody (2 Blots on Right): Peroxidase goat secondary antibody at 1:10,000 for 60 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 50 kDa, 50 kDa for Beta Actin. Other band(s): none.