產(chǎn)品詳情

  • 產(chǎn)品名稱：GranzymeBSubstrate2-pNA

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：KamiyaBiomedical
  • 產(chǎn)品文檔：
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簡(jiǎn)單介紹：
GranzymeBSubstrate2-pNA
詳情介紹：
Purpose Soluble in DMSO and aqueous buffers. We recommend preparing a stock solution in high purity (>99.9%) DMSO, and diluting into aqueous buffer shortly prior to use.
1. Lyse cells in 50 mM Tris-HCl, pH 7.5, 0.3% NP-40, 1.0 mM DTT, at a density of 2 x 10^6 /mL.
2. Assay 0.01 mL cell lysate in a final volume of 0.1 mL. Assay buffer is cell lysis buffer containing 0.2 mM substrate.
3. Incubate at 37°C for 0-3 hrs. Take periodic readings of absorbance at 405 nm.
Sequence Acetyl-Ile-Glu-Pro-Asp-paranitroa nilide, Ac-IEPD-pNA
Specificity Chromogenic substrate for granzyme B.
Purity > 98 % by HPLC
Background Chromogenic paranitroanilide (pNA) peptide substrate for granzyme B. Release of free pNA is monitored by absorbance at 405 nm (epsilon=9,160 M^-1 cm^-1 ). Inhibitory data: Steady-state kinetic parameters for the hydrolysis of Ac-IEPD-pNA by recombinant granzyme B: Kcat = 4.16 S -1 Km x 10 -6 = 57 M Kcat/Km = 66,600 S -1 M -1.
Molecular Weight 634 Da
Comment

Formula: C28H38N6O11
Restrictions For Research Use only
Format Solid
Storage 4 °C