產(chǎn)品詳情

  • 產(chǎn)品名稱：MammalCellProteinExtractionReagent

  • 產(chǎn)品型號：
  • 產(chǎn)品廠商：BosterBio
  • 產(chǎn)品文檔：
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簡單介紹：
MammalCellProteinExtractionReagent
詳情介紹：
Characteristics This reagent is bland To avoid protein degradation by human factor, the preparation should be operated in low temperature and add proper protease inhibitor (Use 1ml for each time Temporary use should add 100mm PMSF 57myl which has high neurotoxicity and absorb easily to skin)
Reagent Preparation

Method 1 For anchorage-dependent cell Remove the cultural solution, wash with PBS, normal saline or cultural solution without serum Add proper lysate and operate with peptide to make the cell and lysate contact fully Usually, the cell will be cleaved after 10 seconds 2 For suspended cell solution without serum Add proper lysate and operate with peptide to blow off the cell Percuss with finger to make the cell cleaved fully Itandrsquo,s not supposed to see clear cell precipitate after full cleavage If too many cells received, subpackage and cleavage 3 After full cleavage 10000-14000 g contrifuge for 3-5 minutes Get the supernatant solution, and do the following operation and do the next steps Dosage of Lysate Plate size / surface area Reagent volume 100mm 500~1000myl 60mm 250~500myl 6-well plate 200~400myl per well 24-well plate 100~200myl per well 96-well plate 50~100myl per well
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Background publications Li, Li, Lu, Tian, Wei: "Allicin ameliorates cognitive deficits ageing-induced learning and memory deficits through enhancing of Nrf2 antioxidant signaling pathways." in: Neuroscience letters, Vol. 514, Issue 1, pp. 46-50, 2012 (PubMed).

Zhou, Chen, Liu, Wang, Xiao, Tao: "Short hairpin ribonucleic acid targeting the telomerase catalytic unit of messenger ribonucleic acid significantly limits the growth of laryngeal squamous cell carcinoma in nude mice." in: The Journal of laryngology and otology, Vol. 122, Issue 5, pp. 513-21, 2008 (PubMed).