產(chǎn)品詳情

  • 產(chǎn)品名稱：RatIgG1IsotypeControl

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：BioLeaf
  • 產(chǎn)品文檔：
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簡(jiǎn)單介紹：
RatIgG1IsotypeControl
詳情介紹：
Clone RG1L
Isotype IgG1
Purification RAT IgG1 Lambda (λ) isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG2a, IgG2b, IgG3 , IgM , and IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum. Light and heavy chain composition has been confirmed.
Sterility Sterile filtered
Research Area Secondary Antibodies
Application Notes

Flow Cytometry Dilution: 1:1000-1:5000

Immunohistochemistry Dilution: User Optimized

Application Note: RAT IgG1 Lambda (λ) isotype control can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user.

Western Blot Dilution: User Optimized

FLISA Dilution: User Optimized

ELISA Dilution: User Optimized

IF Microscopy Dilution: User Optimized
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride,?pH 7.2

Stabilizer: None
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiry Date 12 months