產(chǎn)品詳情
簡(jiǎn)單介紹:
HumanCellArrayII
詳情介紹:
Purpose | Human Cell Array II is provided for immunocytochemical analysis of protein and protein-protein interaction. |
Specificity | Human cell lines (HL-60, Jurkat, K-562, MOLT-4, Raji and THP-1) |
Characteristics |
Human Cell Array II carries 6 different human cell lines, including HL-60, Jurkat, K-562, MOLT-4, Raji and THP-1. Cells are harvested at the log phase of growth. In order to keep the antigens in their native forms, cells were fixed in 4 % paraformaldehyde. The cells were arrayed on a 12-well (5 mm) adhesive coated slide, with each wells surface specifically treated to enhance cellular attachment and to minimize background staining. Each well loaded with approximately 7.5 x 103 cells. A follow-up heat dehydration process was done to ensure the attachment of cells and the stability of cellular proteins. Fixation: 4 % paraformaldehyde Tissue or Cell type HL-60, Jurkat, K-562, MOLT-4, Raji, THP-1 |
Application Notes | Pretreatment with 0.1 % TritonX-100 for 1 minute at room temperature before applying immunocytochemistry is strongly suggested. |
Comment |
We do offer a variety of different lots. Please contact us for further information about available lots. |
Restrictions | For Research Use only |
Storage | 4 °C |
Storage Comment | Human Cell Array II can be stored for up to 3 months at 4 ?°C under desiccate conditions. |
Expiry Date | 3 months |