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  • 產(chǎn)品名稱：Rabbitanti-HumanIg(LightChain),(Chainkappa)Antibody

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簡單介紹：
Rabbitanti-HumanIg(LightChain),(Chainkappa)Antibody
詳情介紹：
Immunogen Purified human Kappa light chain from human Bence Jones proteins.
Isotype IgG
Purification Purified
Alternative Name Immunoglobulin
Background Human Kappa chain is a 25 kDa light chain present on all 5 immunoglobulin's (IgA,IgG, IgM, IgD, and IgE) and is also present in free form. Kappa and Lambda light chains consists of a variable region and a constant region and can be easily be differentiated by the antigenic properties of a constant region. The ratio of Kappa to Lambda chain is 70:30, most of them are bound to heavy chains of immunoglobulin.
Research Area Secondary Antibodies, Virology
Application Notes Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 usingstreptavidinapprox. biotin system or polymer system. FFPE tissue sections require antigen retriever (boiling tissue in 10 mM citrate?pH 6.0 for10-20?minutes, followed by cooling at RT for 15-20?minutes). Recommended positive control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 2.0 mg/mL
Buffer PBS,?pH 7.4 containing 0.05?% Sodium Azide as preservative and 1?% BSA as stabilizer.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Background publications Korkolopoulou, Pangalis, Patsouris, Boussiotis, Kittas: "B-cell lymphoma of large multilobated type: an immunohistochemical study of 8 cases and review of the literature." in: Leukemia & lymphoma, Vol. 13, Issue 1-2, pp. 151-9, 1994 (PubMed).

Schmid, Sargent, Isaacson: "L and H cells of nodular lymphocyte predominant Hodgkin's disease show immunoglobulin light-chain restriction." in: The American journal of pathology, Vol. 139, Issue 6, pp. 1281-9, 1992 (PubMed).

Faure, Tang, Molé, Béné: "Plasma cells producing lambda light chains are predominant in human gut and tonsils. An immunohistomorphometric study." in: American journal of clinical pathology, Vol. 94, Issue 5, pp. 571-5, 1990 (PubMed).