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  • 產(chǎn)品名稱：Mouseanti-HumanIgG2(FabRegion)Antibody(HRP)

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簡單介紹：
Mouseanti-HumanIgG2(FabRegion)Antibody(HRP)
詳情介紹：
Clone HP6014
Isotype IgG1
Purification Affinity Chromatography on Protein A
Background Synonyms: Human Immunoglobulin G2
Research Area Secondary Antibodies
Application Notes ELISA (1:6400 - 1:12800). Immunohistochemistry on frozen sections (1:10 - 1:20).

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS,?pH 7.2, containing 0.05?% ProclinTM300, 0.002?% Methylisothiazolone and 0.002?% Bromonitrodioxane
Preservative ProClin, Methylisothiazolone, Bromonitrodioxane
Precaution of Use This product contains ProClinTM, Methylisothiazolone, Bromonitrodioxane: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Background publications Jefferis, Kumararatne: "Selective IgG subclass deficiency: quantification and clinical relevance." in: Clinical and experimental immunology, Vol. 81, Issue 3, pp. 357-67, 1990 (PubMed).

Hussain, Poindexter, Wistar, Reimer: "Use of monoclonal antibodies to quantify subclasses of human IgG. I. Development of two-site immunoenzymometric assays for total IgG subclass determinations." in: Journal of immunological methods, Vol. 93, Issue 1, pp. 89-96, 1986 (PubMed).

Jefferis, Reimer, Skvaril, de Lange, Ling, Lowe, Walker, Phillips, Aloisio, Wells: "Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: results of an IUIS/WHO collaborative study." in: Immunology letters, Vol. 10, Issue 3-4, pp. 223-52, 1985 (PubMed).