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  • 產(chǎn)品名稱：Mouseanti-Rat(Rattus)ImmunoglobulinG2a(IgG2a)Antibody(FITC)

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  • 產(chǎn)品廠商：Acris-antibodies
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Mouseanti-Rat(Rattus)ImmunoglobulinG2a(IgG2a)Antibody(FITC)
詳情介紹：
Immunogen Purified Rat immunoglobulins from the IR33 - anti horse Ferritin antibody. Spleen cells from immunised BALB/c mice were fused with cells of the PAI-0 mouse myeloma cell line.
Clone MARG2a-1
Isotype IgG1
Purification Affinity Chromatography
Alternative Name IgG2a
Background Synonyms: Rat Immunoglobulin G2a
Research Area Secondary Antibodies
Application Notes Flow Cytometry: 5?μg/mL, use 50 μL of the suggested working dilution to label 10^6 cells in 100 μL.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Comment

Excitation/Emission wavelength: 494 nm/514 nm
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.09?% Sodium Azide and 50?% Glycerol
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Background publications Bazin, Beckers, Urbain-Vansanten, Pauwels, Bruyns, Tilkin, Platteau, Urbain: "Transplantable IgD immunoglobulin-secreting tumors in rat." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 121, Issue 5, pp. 2077-82, 1979 (PubMed).

Shulman, Wilde, K?hler: "A better cell line for making hybridomas secreting specific antibodies." in: Nature, Vol. 276, Issue 5685, pp. 269-70, 1979 (PubMed).

K?hler, Milstein: "Continuous cultures of fused cells secreting antibody of predefined specificity." in: Nature, Vol. 256, Issue 5517, pp. 495-7, 1975 (PubMed).

Bazin, Beckers, Querinjean: "Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c." in: European journal of immunology, Vol. 4, Issue 1, pp. 44-8, 1974 (PubMed).

Bazin, Beckers, Deckers, Moriamé: "Brief communication: Transplantable immunoglobulin-secreting tumors in rats. V. Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in LOU-Wsl rats." in: Journal of the National Cancer Institute, Vol. 51, Issue 4, pp. 1359-61, 1973 (PubMed).