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  • 產(chǎn)品名稱：Mouseanti-Rat(Rattus)ImmunoglobulinG2a(IgG2a)(HeavyChain),(Chaingamma)Antibody

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  • 產(chǎn)品廠商：Acris-antibodies
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Mouseanti-Rat(Rattus)ImmunoglobulinG2a(IgG2a)(HeavyChain),(Chaingamma)Antibody
詳情介紹：
Immunogen Polyclonal rat IgG2a. Spleen cells from immunised BALB/c mice were fused with cells of the mouse PAI-O myeloma cell line.
Clone MARG2a-1
Isotype IgG1
Purification Affinity chromatography
Alternative Name IgG2a
Background Synonyms: Rat Immunoglobulin G2a
Research Area Secondary Antibodies
Application Notes ELISA: 1.25?μg/mL, This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgA (SA021).

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.09?% Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Background publications Bazin, Beckers, Urbain-Vansanten, Pauwels, Bruyns, Tilkin, Platteau, Urbain: "Transplantable IgD immunoglobulin-secreting tumors in rat." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 121, Issue 5, pp. 2077-82, 1979 (PubMed).

Shulman, Wilde, K?hler: "A better cell line for making hybridomas secreting specific antibodies." in: Nature, Vol. 276, Issue 5685, pp. 269-70, 1979 (PubMed).

K?hler, Milstein: "Continuous cultures of fused cells secreting antibody of predefined specificity." in: Nature, Vol. 256, Issue 5517, pp. 495-7, 1975 (PubMed).

Bazin, Beckers, Querinjean: "Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c." in: European journal of immunology, Vol. 4, Issue 1, pp. 44-8, 1974 (PubMed).

Bazin, Beckers, Deckers, Moriamé: "Brief communication: Transplantable immunoglobulin-secreting tumors in rats. V. Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in LOU-Wsl rats." in: Journal of the National Cancer Institute, Vol. 51, Issue 4, pp. 1359-61, 1973 (PubMed).