Application Notes
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/15,000-1/60,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user.
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