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  • 產(chǎn)品名稱：Goatanti-HumanIgM(Fc5muRegion)Antibody(Biotin)

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簡單介紹：
Goatanti-HumanIgM(Fc5muRegion)Antibody(Biotin)
詳情介紹：
Immunogen Human IgM Fc5μ fragment
Purification Immunoaffinity chromatography.
This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Background Synonyms: Human Immunoglobulin M
Research Area Secondary Antibodies, Immunology
Application Notes Suitable for use in Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy aswell as other antibody based assays using streptavidin or avidin conjugates requiringextremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency,high titer and specificity. ELISA: 1:10,000 - 1:50,000. Western Blot: 1:1,000 - 1:5,000. Immunohistochemistry: 1:500 - 1:3,000.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 0.5?mL of deionized water (or equivalent).
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,?pH 7.2 with 10?mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.02?% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -24 °C or below. This antibody is stable for one month at 4 °C as an undiluted liquid.
Background publications Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).