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  • 產品名稱：Rabbitanti-Horse(Equine)IgG(F(ab')2Region)Antibody(HRP)

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  • 產品廠商：Acris-antibodies
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簡單介紹：
Rabbitanti-Horse(Equine)IgG(F(ab')2Region)Antibody(HRP)
詳情介紹：
Immunogen Horse IgG F(ab')2 fragment.
Purification Immunoaffinity chromatography.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Background Synonyms: Horse IgG, Horse Immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for Immunoblotting (Western or Dot blot: use at 1:1,000 - 1:5,000), ELISA,Immunoperoxidase electron microscopy and Immunohistochemistry (use at 1:500 -1:2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lotconsistency. This product has been assayed against 1.0 g of Horse IgG in a standard capture ELISAusing ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30?minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 of thereconstitution concentration is suggested for this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0?mL of deionized water (or equivalent).
Concentration 1.5 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,?pH 7.2 with 10?mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01?% (w/v) Gentamicin Sulfate as preservative.
Preservative Gentamicin sulfate
Precaution of Use This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Background publications Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).