產(chǎn)品詳情

  • 產(chǎn)品名稱(chēng)：Rabbitanti-HumanIgA(Chainalpha)Antibody(HRP)

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  • 產(chǎn)品廠商：Acris-antibodies
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Rabbitanti-HumanIgA(Chainalpha)Antibody(HRP)
詳情介紹：
Immunogen Human IgA alpha alpha chain
Purification Immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Background Synonyms: Human Immunoglobulin A
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/56,000
Western Blot: 1/1,000-1/5,000Immunohistochemistry: 1/500-1/2,500.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0?mL of deionized water or equivalent.
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,?pH 7.2, with 10?mg/mL BSA (IgG and Protease free) as stabilizer and 0.01?% (w/v) Thimerisol as preservative
Preservative Thimerosal (Merthiolate)
Precaution of Use Biohazard Informations: This product contains thimerosal which is hazardous.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50 % glycerol instead of water and then aliquot contents and freeze at -28 °C or below.
Background publications Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).