產(chǎn)品詳情

  • 產(chǎn)品名稱：Rabbitanti-Cow(Bovine)IgG(FcRegion)Antibody(HRP)

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  • 產(chǎn)品廠商：Acris-antibodies
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Rabbitanti-Cow(Bovine)IgG(FcRegion)Antibody(HRP)
詳情介紹：
Immunogen Bovine IgG F(c) fragment
Purification Immunoaffinity chromatography
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads.
Background Synonyms: Bovine Immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electronmicroscopy and immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0?μg of Bovine IgG in a standard capture ELISAusing ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30?minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggestedfor this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0?mL of deionized water or equivalent.
Concentration 1.5 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,?pH 7.2, containing 10?mg/mL Polyethylene Glycol (PEG-8000) as a stabilizer and 0.01?% (w/v) Gentamicin Sulfate as a preservative
Preservative Gentamicin sulfate
Precaution of Use This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage 4 °C
Storage Comment Store vial at 2-8 °C prior to restoration. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -28 °C or below.
Background publications Bixler, Pillai: "Augmentation by interleukins of the antibody response to a conjugate vaccine against Haemophilus influenzae b." in: Advances in experimental medicine and biology, Vol. 303, pp. 185-90, 1992 (PubMed).

Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).