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  • 產(chǎn)品名稱：Goatanti-HumanIgG(F(ab')2Region)Antibody(HRP)

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  • 產(chǎn)品廠商：Acris-antibodies
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簡單介紹：
Goatanti-HumanIgG(F(ab')2Region)Antibody(HRP)
詳情介紹：
Immunogen Human IgG F(ab')2 fragment
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Background Synonyms: Human Immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electronmicroscopy and immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring extremely low background levels, absence of F(c) mediatedbinding, lot-to-lot consistency, high titer and specificity. Recommended dilutions: ELISA 1:10,000 - 1:40,000
Western blot 1:1,000 - 1:5,000Immunohistochemistry 1:500 - 1:2,500

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 0.5?mL of deionized water (or equivalent).
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,?pH 7.2, containing 10?mg/mL BSA, IgG and Protease free, and 0.01?% (w/v) Gentamicin Sulfate
Preservative Gentamicin sulfate
Precaution of Use This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50 % glycerol instead of water and then aliquot contents and freeze at -28 °C or below.
Background publications Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).