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  • 產(chǎn)品名稱：Rabbitanti-Cow(Bovine)IgG(WholeMolecule)Antibody(HRP)

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  • 產(chǎn)品廠商：Acris-antibodies
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簡單介紹：
Rabbitanti-Cow(Bovine)IgG(WholeMolecule)Antibody(HRP)
詳情介紹：
Immunogen Bovine IgG whole molecule.
Purification Immunoaffinity Chromatography.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads.
Background Synonyms: Bovine Immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for Immunoblotting (Western blot: 1/1,000-1/10,000 or Dot blot), ELISA (1/10,000-1/50,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiringlot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0?μg of Bovine IgG in astandard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30?minutes at room temperature. A working dilution of 1:1,000 to 1:5,000of the reconstitution concentration is suggested for this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0?mL of deionized water (or equivalent).
Concentration 2.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,?pH 7.2 with 10?mg/mL Polyethylene Glycol (PEG-8000) as stabilizer and 0.01?% (w/v) Gentamicin Sulfate as preservative.
Preservative Gentamicin sulfate
Precaution of Use This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage 4 °C
Storage Comment Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Background publications Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).