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簡單介紹:
Mouseanti-HumanIgG1(FcRegion)Antibody
詳情介紹:
Immunogen Purified Human IgG1
Clone 8c-6-39 (HP6091)
Isotype IgG2a
Purification Affinity Chromatography on Protein A
Background Synonyms: Human Immunoglobulin G1
Research Area Secondary Antibodies
Application Notes ELISA. Immunohistochemistry on frozen sections. Not suitable for paraffin sections. Immunodiffusion.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.09?% Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Background publications Jefferis, Reimer, Skvaril, de Lange, Goodall, Bentley, Phillips, Vlug, Harada, Radl: "Evaluation of monoclonal antibodies having specificity for human IgG subclasses: results of the 2nd IUIS/WHO collaborative study." in: Immunology letters, Vol. 31, Issue 2, pp. 143-68, 1992 (PubMed).

Jefferis, Kumararatne: "Selective IgG subclass deficiency: quantification and clinical relevance." in: Clinical and experimental immunology, Vol. 81, Issue 3, pp. 357-67, 1990 (PubMed).

Hussain, Poindexter, Wistar, Reimer: "Use of monoclonal antibodies to quantify subclasses of human IgG. I. Development of two-site immunoenzymometric assays for total IgG subclass determinations." in: Journal of immunological methods, Vol. 93, Issue 1, pp. 89-96, 1986 (PubMed).

Jefferis, Reimer, Skvaril, de Lange, Ling, Lowe, Walker, Phillips, Aloisio, Wells: "Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: results of an IUIS/WHO collaborative study." in: Immunology letters, Vol. 10, Issue 3-4, pp. 223-52, 1985 (PubMed).

Jefferis, Mathews: "Studies of human IgD myeloma proteins. Proteolytic digestion patterns." in: Immunochemistry, Vol. 14, Issue 3, pp. 171-8, 1977 (PubMed).