產(chǎn)品詳情
簡單介紹:
Rabbitanti-HumanIgG(F(ab')2Region)Antibody(FITC)
詳情介紹:
Immunogen | Human IgG F(ab')2 fragment |
Characteristics | Molar Ratio: 2.5 moles FITC per mole of Rabbit IgG |
Purification | Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities |
Background | Synonyms: Human Immunoglobulin G |
Research Area | Immunology, Secondary Antibodies |
Application Notes |
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring lot-to-lot consistency. Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user. |
Comment |
Absorption / Emission: 495 nm / 528 nm |
Restrictions | For Research Use only |
Reconstitution | Restore with 1.0?mL of deionized water or equivalent. |
Concentration | 1.0 mg/mL |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,?pH 7.2, containing 10?mg/mL BSA (IgG and Protease free) as stabilizer, and 0.01?% (w/v) Sodium Azide as preservative |
Preservative | Sodium azide |
Precaution of Use | WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
Handling Advice |
Product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. |
Storage | 4 °C/-20 °C |
Storage Comment | Store vial at 2-8 °C prior to restoration. This product is stable for one month at 2-8 °C as an undiluted liquid. For extended storage reconstitute product with 50 % glycerol instead of water and then aliquot contents and freeze at -28 °C or below. |
Background publications |
The, Feltkamp: "Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation." in: Immunology, Vol. 18, Issue 6, pp. 865-73, 1970 (PubMed).
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