產(chǎn)品詳情
簡單介紹:
Rabbitanti-HumanIgG(F(ab')2Region)Antibody(FITC)
詳情介紹:
Immunogen | Human IgG F(ab')2 fragment |
Characteristics | Molar Ratio: 2.8 moles DTAF per mole of Rabbit IgG |
Purification | Prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities |
Background | Synonyms: Human Immunoglobulin G |
Research Area | Immunology, Secondary Antibodies |
Application Notes |
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring lot-to-lot consistency. Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user. |
Comment |
Absorption / Emission: 495 nm / 528 nm |
Restrictions | For Research Use only |
Reconstitution | Restore with 1.0?mL of deionized water (or equivalent). |
Concentration | 2.0 mg/mL |
Buffer | 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride,?pH 7.4, 10?mg/mL Bovine Serum Albumin (BSA) IgG and Protease free, 0.01?% (w/v) Thimerisol |
Preservative | Thimerosal (Merthiolate) |
Precaution of Use | Biohazard Informations: This product contains thimerosal which is hazardous. |
Handling Advice |
Product is photosensitive and should be protected from light. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. |
Storage | 4 °C/-20 °C |
Storage Comment | Store vial at -20 °C prior to opening. For extended storage aliquot contents and freeze at -20 °C or below. |
Background publications |
The, Feltkamp: "Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation." in: Immunology, Vol. 18, Issue 6, pp. 865-73, 1970 (PubMed).
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