產(chǎn)品詳情

  • 產(chǎn)品名稱(chēng)：MammalTissueProteinExtractionReagent

  • 產(chǎn)品型號(hào)：
  • 產(chǎn)品廠商：BosterBio
  • 產(chǎn)品文檔：
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MammalTissueProteinExtractionReagent
詳情介紹：
Characteristics This reagent is bland Add a small amount of detergent when cytolyse, and the detergent can be dialyzed Additionally, this reagent has no effect on the detection of standard protein
Reagent Preparation

Method 1 Place the fresh tissue into pre-cooling normal saline and wash several times to clean the blood on the surface Weigh the tissue samples, cut into pieces before adding to a glass homogenizer 2 Homogenize tissue samples in 1ml of extraction reagent per 01g of tissue (Increase the amount of extraction reagent if the samples were not lysed completely, Reduce the amount of extraction reagent if need protein samples with high concentration) Add PMSF (100mmol/ml) before using and dilute to 1m mol/ml 3 Homogenization using a glass homogenizer until the tissue samples were lysed completely 4 Centrifuge for 3-5 minutes at 10000-14000 g Get the supernatant solution, and do the next steps
Restrictions For Research Use only
Format Liquid
Storage 4 °C
Background publications Gui, Xiong, Yang, Li, Huang: "Effects of acupuncture on LIF and IL-12 in rats of implantation failure." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 67, Issue 5, pp. 383-90, 2012 (PubMed).