產(chǎn)品詳情
簡(jiǎn)單介紹:
MouseIgGIsotypeControl
詳情介紹:
Isotype | IgG |
Fragment | Whole molecule |
Characteristics | Mouse IgG2b control (Whole Molecule), is suitable to be used as control, standard, blocking agent, or coating protein in a variety of assays, including ELISA, immunobloting (Dot blot and Western blot), immunoprecipitation, immunodiffusion, and immunoelectrophoresis. It may also be used as antigen or ligand in immunochemical conjugation reaction. The Mouse IgG2b control (Whole Molecule)is highly purified from normal mouse ascites through Protein A affinity column and produced from the hybridoma resulting from fusion of SP2/0-Ag14 myeloma and B-lymphocytes. |
Purification | Protein A affinity column |
Research Area | Immunology, Secondary Antibodies |
Application Notes | Each Investigator should determine their own optimal working dilution for specific applications. |
Restrictions | For Research Use only |
Format | Lyophilized |
Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to make a concentration of 1 mg/mL. |
Concentration | 1 mg/mL |
Buffer | PBS, pH 7.4, containing 0.02 % sodium azide. |
Preservative | Sodium azide |
Precaution of Use | WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
Handling Advice | Avoid repeated freezing and thawing cycles. |
Storage | 4 °C/-20 °C |
Storage Comment | The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below. |