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  • 產(chǎn)品名稱：Goatanti-HumanIgA,IgG,IgM(WholeMolecule)Antibody(HRP)

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  • 產(chǎn)品廠商：Acris-antibodies
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簡單介紹：
Goatanti-HumanIgA,IgG,IgM(WholeMolecule)Antibody(HRP)
詳情介紹：
Immunogen Human IgG, IgA and IgM whole molecules
Purification This product was prepared from polyspecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Background Synonyms: Human IgA, Human IgG, Human IgM
Application Notes Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electronmicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring lot-to-lot consistency. Recommended dilutionsELISA 1/10,000-1/70,000. Western blot 1/1,000-1/5,000. Immunohistochemistry 1/500-1/2,500.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0?mL of deionized water (or equivalent).
Concentration 2.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride,?pH 7.2 containing 10?mg/mL BSA (IgG and Protease free) as stabilizer and 0.01?% (w/v) Gentamicin Sulfate as a preservative.
Preservative Gentamicin sulfate
Precaution of Use This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Product cited in: Ramm, Hartmann, Tudorache, Haverich, Hilfiker: "No evidence for αGal epitope transfer from media containing FCS onto human endothelial cells in culture." in: Xenotransplantation, Vol. 22, Issue 5, pp. 345-55, 2015 (PubMed).

von Rhein, Hunfeld, Ludwig: "Serologic evidence for effective production of cytolysin A in Salmonella enterica serovars typhi and paratyphi A during human infection." in: Infection and immunity, Vol. 74, Issue 11, pp. 6505-8, 2006 (PubMed).

Background publications Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).