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  • 產(chǎn)品名稱：Donkeyanti-GoatIgGAntibody(AlkalinePhosphatase(AP))

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Donkeyanti-GoatIgGAntibody(AlkalinePhosphatase(AP))
詳情介紹：
Immunogen Purified IgG from a Sheep serum pool
Isotype IgG
Cross-Reactivity Sheep (Ovine)
Purification Affinity Chromatography
Research Area Immunology, Secondary Antibodies
Application Notes Western blot. ELISA: 1/2000-1/5000. Immunohistochemistry on Frozen Sections.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer Tris Buffered Saline,?pH 8.0 containing 0.01?% Sodium Azide, 1?% BSA, 50?% Glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Background publications Yekta, Goddeeris, Vanrompay, Cox: "Immunization of sheep with a combination of intiminγ, EspA and EspB decreases Escherichia coli O157:H7 shedding." in: Veterinary immunology and immunopathology, Vol. 140, Issue 1-2, pp. 42-6, 2011 (PubMed).

Singh, Kurfurst, Nizard, Schnebert, Perrier, Tobin: "Melanin transfer in human skin cells is mediated by filopodia--a model for homotypic and heterotypic lysosome-related organelle transfer." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 10, pp. 3756-69, 2010 (PubMed).

Turner, Gonzalez-Juarrero, Ellis, Basaraba, Kipnis, Orme, Cooper: "In vivo IL-10 production reactivates chronic pulmonary tuberculosis in C57BL/6 mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 11, pp. 6343-51, 2002 (PubMed).

Singh, Cattaneo, Billeter: "A recombinant measles virus expressing hepatitis B virus surface antigen induces humoral immune responses in genetically modified mice." in: Journal of virology, Vol. 73, Issue 6, pp. 4823-8, 1999 (PubMed).

Tedla, Wang, McNeil, Di Girolamo, Hampartzoumian, Wakefield, Lloyd: "Regulation of T lymphocyte trafficking into lymph nodes during an immune response by the chemokines macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 10, pp. 5663-72, 1998 (PubMed).

Lucas, Forman, Samuels, Granner: "Specificity of a retinoic acid response element in the phosphoenolpyruvate carboxykinase gene promoter: consequences of both retinoic acid and thyroid hormone receptor binding." in: Molecular and cellular biology, Vol. 11, Issue 12, pp. 6343, 1991 (PubMed).