產(chǎn)品詳情
簡(jiǎn)單介紹:
Mouseanti-HumanIgM(HeavyChain),(Chainmu)Antibody
詳情介紹:
Immunogen | Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. |
Clone | M15-8 |
Isotype | IgG1 |
Purification | Affinity chromatography on Protein A |
Background | Synonyms: Human Immunoglobulin M |
Research Area | Secondary Antibodies, Immunology |
Application Notes |
Flow Cytometry: 20?μg/mL. Immunohistochemistry on frozen sections: 20?μg/mL. ELISA: 50?μg/mL as coating Ab. Immunoprecipitation. Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user. |
Restrictions | For Research Use only |
Concentration | 3.71 mg/mL |
Buffer | PBS,?pH 7.4 containing 0.09?% Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
Handling Advice | Avoid repeated freezing and thawing. |
Storage | 4 °C/-20 °C |
Storage Comment | Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer. |
Background publications |
Cragg, Zhang, French, Glennie: "Analysis of the interaction of monoclonal antibodies with surface IgM on neoplastic B-cells." in: British journal of cancer, Vol. 79, Issue 5-6, pp. 850-7, 1999 (PubMed).
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