產(chǎn)品詳情
簡單介紹:
Donkeyanti-HumanIgG(WholeMolecule)Antibody(PE)
詳情介紹:
Immunogen | Human IgG whole molecule. |
Purification |
Immunoaffinity chromatography. This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. |
Background | Synonyms: Human Immunoglobulin G |
Research Area | Immunology, Secondary Antibodies |
Application Notes |
Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Recommended Dilutions: The maximum amount of reagent required to stain 1 x 10^6 cellsin Flow Cytometry is approximately 1.0 g of antibody conjugate. Lesser amounts ofreagent may be sufficient for staining. As a general guideline dilutions of 1:50 to 1:200should be suitable for most applications. Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user. |
Comment |
Absorption / Emission: 490 nm, 545 nm and 565 nm / 580 nm |
Restrictions | For Research Use only |
Format | Liquid |
Reconstitution | Restore with 1.0?mL of deionized water (or equivalent). |
Concentration | 0.5 mg/mL |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,?pH 7.2 with 0.02?% (w/v) Sodium Azide as preservative and 10?mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer. |
Preservative | Sodium azide |
Precaution of Use | WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
Handling Advice |
Product is photosensitive and should be protected from light. Avoid repeated freeze-thaw cycles. |
Storage | 4 °C/-20 °C |
Storage Comment | Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -28 °C for longer. |