產(chǎn)品詳情
簡單介紹:
Mouseanti-HumanIg(LightChain),(Chainkappa)Antibody
詳情介紹:
Immunogen | Human IgG-kappa myeloma protein |
Clone | TB28-2 |
Isotype | IgG1 |
Cross-Reactivity (Details) | Species reactivity (tested):Human |
Purification | Protein-A affinity chromatography |
Alternative Name | Immunoglobulin |
Background | Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5?kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30. |
Research Area | Secondary Antibodies, Virology |
Application Notes | Optimal working dilution should be determined by the investigator. |
Restrictions | For Research Use only |
Concentration | 1.0 mg/mL |
Buffer | PBS,?pH ~7.4, 15 mM Sodium Azide |
Preservative | Sodium azide |
Precaution of Use | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Handling Advice | Avoid repeated freezing and thawing. |
Storage | 4 °C/-20 °C |
Storage Comment | Storer the antibody at 2-8 °C for up to one month or (in aliqouts) at -20 °C for longer. |
Background publications |
Davis, Karl, Swan, Unlu, Goldberg: "Capabilities and limitations of pupil-plane filters for superresolution and image enhancement." in: Optics express, Vol. 12, Issue 17, pp. 4150-6, 2009 (PubMed).
Kiyotaki, Cooper, Bertoli, Kearney, Kubagawa: "Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 12, pp. 4150-8, 1987 (PubMed). |
Product cited in: |
Jourdan, Caraux, De Vos, Fiol, Larroque, Cognot, Bret, Duperray, Hose, Klein: "An in vitro model of differentiation of memory B cells into plasmablasts and plasma cells including detailed phenotypic and molecular characterization." in: Blood, Vol. 114, Issue 25, pp. 5173-81, 2009 (PubMed).
B?ttcher, Ritgen, Buske, Gesk, Klapper, Hoster, Hiddemann, Unterhalt, Dreyling, Siebert, Kneba, Pott et al.: "Minimal residual disease detection in mantle cell lymphoma: methods and significance of four-color flow cytometry compared to consensus IGH-polymerase chain reaction at initial staging and for ..." in: Haematologica, Vol. 93, Issue 4, pp. 551-9, 2008 (PubMed). Karandikar, Aquino, McKenna, Kroft: "Transient myeloproliferative disorder and acute myeloid leukemia in Down syndrome. An immunophenotypic analysis." in: American journal of clinical pathology, Vol. 116, Issue 2, pp. 204-10, 2001 (PubMed). Nakamura, Kubagawa, Cooper: "Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 18, pp. 8522-6, 1992 (PubMed). |