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  • 產(chǎn)品名稱：Rabbitanti-HumanIgG(F(ab')2Region)Antibody(Biotin)

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  • 產(chǎn)品廠商：Acris-antibodies
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簡單介紹：
Rabbitanti-HumanIgG(F(ab')2Region)Antibody(Biotin)
詳情介紹：
Immunogen Human IgG F(ab')2 fragment.
Characteristics Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.
Purification Prepared from monospecific antiserum by Immunoaffinity Chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Background Synonyms: Human Immunoglobulin G
Research Area Immunology, Secondary Antibodies
Application Notes Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0?μg of Human IgG in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30?minutes atroom temperature. A working dilution of 1/30,000 to 1/120,000 of the reconstitution concentration issuggested for this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0?mL of deionized water (or equivalent).
Concentration 2.0 mg/mL
Buffer 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride,?pH 7.4, with 10?mg/mL BSA (IgG and Protease free) as stabilizer and 0.01?% (w/v) Thimerisol as preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use Biohazard Informations: This product contains thimerosal which is hazardous.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Background publications Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).