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  • 產(chǎn)品名稱：Mouseanti-Rat(Rattus)IgG1(HeavyChain),(Chaingamma)Antibody(FITC)

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  • 產(chǎn)品廠商：Acris-antibodies
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Mouseanti-Rat(Rattus)IgG1(HeavyChain),(Chaingamma)Antibody(FITC)
詳情介紹：
Immunogen Rat IR27 IgG1 myeloma. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Clone MARG1-2
Isotype IgG1
Purification Affinity Chromatography
Background Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17?mg/mL in ***** blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions, to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10e11 variants. Immunoglobulins, or antibodies, are a complex, heterogeneous mixture of proteins that exhibit two fundamental types of structural variation. Subtle structural differences in their antigen combining sites, or variable regions, account for their unique antigen binding specificities.Synonyms: Rat Immunoglobulin G1
UniProt P20759
Research Area Secondary Antibodies
Application Notes Flow Cytometry: 5?μg /mL, use 50 μL of the suggested working dilution to label 10^6 cells in 100 μL.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Comment

Excitation/Emission wavelength: 494 nm/514 nm
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.09?% Sodium Azide and 50?% Glycerol
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Background publications Pelegrí, Castell, Serra, Rabanal, Rodríguez-Palmero, Castellote, Franch: "Prevention of adjuvant arthritis by the W3/25 anti-CD4 monoclonal antibody is associated with a decrease of blood CD4(+)CD45RC(high) T cells." in: Clinical and experimental immunology, Vol. 125, Issue 3, pp. 470-7, 2001 (PubMed).